There are two major types of skeletal keratan sulphates.

On the polylactose nature of chondroitin and keratan sulphates.

The recognition that keratan and chondroitin sulphates are based on the same polylactose backbone simplifies the study of heteroduplex formation between them, suggests insights into structure-function relationships in tissues, and permits new definitions of the linkage regions.

Constant and variable domains of different disaccharide structure in corneal keratan sulphate chains.

Four peptidokeratan sulphate fractions of different Mr and degree of sulphation were cut from the pig corneal keratan sulphate distribution spectrum. After exhaustive digestion with keratanase, the fragments were separated on DEAE-Sephacel and Bio-Gel P-10 and analysed for their Mr, degree of sulphation and amino sugar and neutral sugar content. It was found that every glycosaminoglycan chain i...

High-field n.m.r. studies of keratan sulphates. 1H and 13C assignments of keratan sulphate from shark cartilage.

Keratan sulphate was extracted from a shark/whale cartilage preparation and examined by 400 MHz 1H- and 100 MHz 13C-n.m.r. spectroscopy. Assignment of the majority of the resonances was facilitated by two-dimensional 13C-1H correlation by using a modified COLOC procedure and a COSY-45 experiment. The spectra are consistent with an N-acetyl-lactosamine repeating unit that is predominantly sulpha...

The Keratan Sulphates: Structural Investigations Using Nmr Spectroscopy

Lactate production and utilization by a differentiated culture of sheep thyroid cells.

Dietrich, 1976): however, background staining is high and variable. Alcian Blue with 0.05 M-MgCI, provided effective labelling of all cartilage glycosaminoglycans, including trace amounts of hyaluronate. The hyaluronate did not form a complex with Alcian Blue either in the absence of MgCI, or at molarities greater than 0.1. In the described scheme, hyaluronate was resolved from chondroitin and ...